Journal: Translational Psychiatry

Article Title: CCK2R regulates METH-induced CPP acquisition within VTA-BLA-BNST circuit in male mice

doi: 10.1038/s41398-026-03982-y

Figure Lengend Snippet: (A) Schematic of METH CPP procedure. (B) The expression of CCK1R mRNA. n = 5 mice. (C) The expression of CCK2R mRNA. BLA: Unpaired t test, t = 4.180, P = 0.0031, n = 5 mice. (D) The expression of CCKR mRNA in BLA after METH training. n = 5 mice. (E) Top: Representative immunoblots of CCK1R. Bottom: Relative amounts of CCK1R quantified by densitometry, in BLA. Unpaired t test, t = 0.317, P = 0.759, n = 5 mice. (F) Top: Representative immunoblots of CCK2R. Bottom: Relative amounts of CCK2R quantified by densitometry, in BLA. Unpaired t test, t = 2.620, P = 0.031, n = 5 mice. (G) Schematic of METH CPP procedure. (H) Schematic diagram of virus injection in BLA. Scale bar=200μm . (I, J) Heat map and score bar chart of METH CPP. Two-way RM ANOVA, F treatment (3,44) = 21.94, P <0.001; Tukey’s post hoc comparison, CCK2R-KO + METH vs Vector+METH: P <0.001, n = 12 mice. (K, L) In vitro electrophysiology: AP spiking patterns evoked by 400 pA current injections and spike numbers and thresholds for AP firing. Two-way RM ANOVA ANOVA, F treatment (3,44) = 31.20, P <0.001; Tukey’s post hoc comparison, Vector+Saline vs Vector+METH: P <0.001, CCK2R-KO + METH vs Vector+METH: P <0.001, n = 12 cells from 4 mice. (M, N) In vivo electrophysiology: AP spiking patterns and bar chart of AP frequency. Ordinary one-way ANOVA, F treatment (3,44) = 12.32, P <0.001; Tukey’s post hoc comparison, Vector+Saline vs Vector+METH: P <0.001, CCK2R-KO + METH vs Vector+METH: P <0.001, n = 12 cells from 3 mice. All data are means ± SEM, * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Membranes were then blocked for 1 h using 5% skim milk in TBS before overnight incubation at 4 °C with primary antibodies: mouse GAPDH (1:10,000 dilution, ABclonal, AC033), CCK1R (1:150, Santa Cruz, sc-514303) or CCK2R (1:150, Santa Cruz, sc-166690).

Techniques: Expressing, Western Blot, Virus, Injection, Comparison, Plasmid Preparation, In Vitro, Saline, In Vivo

Journal: Frontiers in Oncology

Article Title: CCKAR is a biomarker for prognosis and asynchronous brain metastasis of non-small cell lung cancer

doi: 10.3389/fonc.2022.1098728

Figure Lengend Snippet: Expression of CCKAR in NSCLC. (A) The expression of CCKAR in NSCLC was detected by IHC. Patients with NSCLC were divided into subsets with low or high CCKAR expression. (B) Expression of CCK-8 in NSCLC was detected with IHC and representative images were shown. (C) CCKAR expression in 12 pairs of NSCLC and para-tumor tissues was detected with qRT-PCR. The statistical significance was evaluated by paired t test. (D) Asynchronous BM percentages of patients with low and high CCKAR were calculated and compared with chi-square method. Patients with high CCKAR in NSCLC primary tumor were much more susceptible to BM.

Article Snippet: The primary antibody of CCKAR (1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or CCK-8 (1:100, Abcam, ab27441, UK) was used to incubate the specimens overnight at 4°, and the corresponding secondary antibody labelled with streptavidin-biotin-peroxidase reagent (Beyotime, Beijing, China) was administrated to incubate the specimens for 30 minutes at room temperature.

Techniques: Expressing, CCK-8 Assay, Quantitative RT-PCR